Detection of bovine tuberculosis using a genosensor (nano-genosensor) based on fluorescent semiconductor nanoparticles
AssigneeUniversidad San Sebastian
InventorsMaría Ligia INOSTROZA CONCHA, Patricio OYARZUN CAYO, Victor DIAZ GARCIA
Life of this patent
Estimating the term…
Abstract
A method for detection of Mycobacterium bovis based on fluorescent semiconducting nanoparticle nano-genosensors (Quantum Dots, QDs), genomic probes and a quencher is provided. Upon exposure of the nano-genosensor to M. bovis DNA, the quencher is displaced, and the DNA binds to the probe bond to the fluorescent semiconductor nanoparticle composed of Cadmium Telluride (CdTe), thus causing an increase in fluorescence (turn-on). The photonic nano-genosensor emits fluorescence proportional to the amount of Mycobacterium bovis DNA present in a sample.
Claims10
A nano-genosensor for detecting the presence of Mycobacterium bovis, CHARACTERIZED in that it comprises:
- a. fluorescent semiconductor nanoparticles;
- b. genomic probes;
- and
- c. a fluorescence quencher coupled with the sequence complementary to the genomic probe sequence;
- wherein the nanoparticles are functionalized with the genomic probe.
Dependencies are inferred from the claim text.