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US 2024/0392302 A1Published application
Method for detecting and quantifying rna
AssigneeFluorescence Diagnosis (Shanghai) Biotech Company
InventorDasheng ZHANG
Life of this patent
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Abstract
An aptamer nucleic acid molecule, a complex containing the aptamer and a small fluorophore molecule, a method for detecting intracellular or extracellular RNA, DNA or other target molecules using the aptamer nucleic acid molecule, and a kit containing the aptamer. The aptamer of the present disclosure can specifically bind with a small fluorophore molecule, and can significantly improve the fluorescence intensity thereof under the excitation of light of suitable wavelength.
Claims22
A nucleic acid aptamer molecule, comprising following nucleotide sequences (a), (b) or (c):
- (a):
- a nucleotide sequence
- N1CUCCAGGUGN11-N12-N13GUAACGGACUUAGAUCCACUGUACGN39, wherein N1, N11, N12, N13 and N39 represent nucleotide fragments greater than or equal to 1 in length, and at least one base pair in N1 and N39 nucleotide sequences forms a complementary pair, and at least one base pair in N11 and N13 nucleotide sequences forms a complementary pair;
- (b):
- a nucleotide sequence with an identity of at least 71% to the nucleotide sequence limited by (a);
- and
- (c):
- a nucleic acid aptamer molecule derived from (a) at a position not including N1, N11, N12, N13 and N39 in the nucleotide sequence limited by (a), with substitution, missing and/or addition of one or several nucleotides, and having aptamer functions.
5 more elements
19-22. (canceled)
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Dependencies are inferred from the claim text.