Methods and compositions for inducing tumor cell death
AssigneeStitch Bio, LLC
InventorAnthony P. Shuber
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Abstract
The disclosure provides methods and compositions that employ gene editing systems to enable cells to express guide RNAs. Gene editing systems specifically target fusions in tumor DNA to introduce a coding sequence that is expressed by tumor cells as a guide RNA that targets known repetitive elements in the human genome in tumor cells. The CRISPR-like systems are expressed in the tumor cells and cleave the tumor DNA at the known repetitive elements thereby inducing tumor cell death.
Claims35
A method of inducing tumor cell death, the method comprising:
- identifying one or more fusions in tumor DNA obtained from a subject;
- delivering to said subject a gene editing system, a first vector comprising DNA encoding a guide RNA (gRNA) capable of hybridizing with a common region within a repetitive sequence present in the human genome, and a second vector comprising DNA encoding a Cas-related endonuclease;
- wherein said gene editing system targets one or more of said fusions;
- and wherein expression of said gRNA and said Cas-related endonuclease result in cleavage of said tumor DNA.
A recombinant DNA molecule adapted for expression of a guide RNA (gRNA) for association with a Cas-related endonuclease, comprising a nucleic acid molecule encoding a gRNA that is complementary to a repetitive element in the human genome and an expression control sequence operatively linked thereto.
Dependencies are inferred from the claim text.