Multiplexed Single Cell Gene Expression Analysis Using Template Switch and Tagmentation
AssigneeIllumina, Inc.
InventorsFiona Kaper, Jian-Bing Fan, Neeraj Salathia, Gordon M. Cann, Arash Jamshidi, Alex Aravanis
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Abstract
Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).
Claims21
-20. (canceled)
A solid support comprising immobilized first-strand synthesis primers, wherein each first-strand synthesis primer comprises a unique molecular identifier (UMI) and a capture sequence, wherein the first-strand synthesis primers are immobilized in an ordered pattern on the surface of the solid support.
A solid support comprising immobilized first-strand synthesis primers, wherein each first-strand synthesis primer comprises:
- a. an index sequence;
- b. a sample barcode;
- c. a sequencing adapter sequence;
- and
- d. a capture sequence,
- wherein the first-strand synthesis primers are immobilized in an ordered pattern on the surface of the solid support.
A reaction vessel comprising:
- a. a TSO comprising a template-switching primer sequence;
- and
- b. a solid support comprising a plurality of immobilized oligonucleotides each comprising:
- i. a sample barcode;
- ii. a UMI;
- and
- iii. the template-switching primer sequence.
Dependencies are inferred from the claim text.