Enzymes with improved thermostability for the degradation of plastic products
OwnerDaniel Eduardo RODRÍGUEZ CHAMORROinventor
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Abstract
The present invention is located in the field of biotechnology, it refers to the creation of recombinant enzymes with improved thermostability, from mutations made in the Thermobifida fusca cutinase. Likewise, the present invention refers to the use of recombinant enzymes in the degradation of aromatic and semi-aromatic polymers and their oligomers, such as plastic.
Claims22
A recombinant enzyme of the esterase group, cutinases and lipases having at least 70% sequence identity of SEQ ID Nr 1 and a thermostability value of 68° C. to 99° C.
An in vitro method to produce the recombinant enzyme of sequences SEQ ID Nr 1 to SEQ ID Nr 7, comprising the steps of:
- a. culturing host cells where the coding sequence was previously inserted;
- and
- b. recovering said enzyme.
The use of recombinant enzymes of sequences SEQ ID Nr 1 to SEQ ID Nr 7 for the degradation of aromatic polymers, semi-aromatics and its oligomers, such as plastics.
Dependencies are inferred from the claim text.