Methods and Compositions for the Labeling and Selection of Antigen-Specific T-Cells
AssigneeThe Regents of the University of California
InventorsChristopher S. Seet, Jocelyn T. Kim, Gay M. Crooks
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Abstract
The current disclosure provides for the simultaneous isolation and selection of antigen-specific T cells by providing engineered proteins, cell lines, and viruses that have a peptide-major histocompatibility complex (pMHC) on the surface of the virus that facilitates transduction of the T cell that, through the T cell receptor (TCR), specifically binds to the peptide in the pMHC complex. Accordingly, aspects of the disclosure relate to proteins, cells, and viral particles that can achieve these methods.
Claims94
A viral particle comprising a nucleic acid encoding a single chain trimer (SCT), a SVGmu protein and/or nucleic acid encoding a SVGmu protein, and a nucleic acid encoding a detection gene, wherein the viral particle comprises a nucleic acid encoding a fusion protein comprising the SCT and SVGmu.
A viral particle comprising a nucleic acid encoding a single chain trimer (SCT), a SVGmu protein, and a nucleic acid encoding a detection gene, wherein the detection gene encodes for a fusion protein comprising the detection gene and a viral protein, and wherein the viral protein comprises a matrix protein (MA), nucleocapsid protein (NC), or viral protein R (VPR).
A chimeric protein comprising at least a portion of a major histocompatibility complex (MHC) polypeptide and a viral protein or a fragment thereof.
A chimeric protein comprising a detection gene and a viral protein or a fragment thereof.
A chimeric protein comprising at least a portion of a major histocompatibility complex and a transmembrane domain.
A viral vector comprising one or more of a MHC polypeptide, SVGmu, mutant VSV-G, and a detection gene.
A host cell comprising all or a portion of a MHC polypeptide, wherein the host cell is a viral packaging cell line.
A host cell comprising one or more nucleic acids encoding all or a portion of a MHC polypeptide, wherein the host cell is a viral packaging cell line.
A viral particle comprising all or a portion of a MHC polypeptide linked to the surface of the viral envelope.
The viral particle of any one of claims 60-77, wherein a peptide is linked to the MHC polypeptide.
Dependencies are inferred from the claim text.