Gene sequence construct for gene therapy for hiv infection

Abstract

The present invention relates to a gene sequence construct for gene therapy for HIV infection. The gene sequence construct is constructed by means of sequentially connecting, via a coding sequence of a linker polypeptide, a gene coding sequence of a variable region scFv in each light chain and heavy chain of a monoclonal antibody against antigens at different binding sites involved in the different steps of HIV infection of a human CD4+ T cell, a gene coding sequence of a variable region scFv in each light chain and heavy chain of a monoclonal antibody bound to a human CD4 receptor site, a gene coding sequence of an Fc fragment in a human IgG constant region, and a gene coding sequence of a polypeptide for inhibiting the fusion of HIV and a CD4+ T cell membrane, and by placing the connected gene coding sequences downstream of a promoter and a secretion signal peptide coding sequence, thereby expressing a single-gene-encoded secretion-type antibody-like protein molecule. The recombinant single-gene construct can be conveniently introduced into a target tissue cell via a viral vector, and the expressed secretion-type antibody-like protein molecule has multi-antigen tropism, can realize the efficient and broad-spectrum blockade of the HIV infection process on a human CD4+ T cell by means of binding to multiple binding sites involved in the different steps of HIV infection of the human CD4+ T cell, and effectively avoids the loss of the ability to inhibit an HIV infection due to an HIV escape mutation, thereby achieving a long-term or even permanent treatment effect on HIV infection by means of a single injection.

Dependencies are inferred from the claim text.