Microfluidic device

A microfluidic device for thermocycling of a reaction mixture for a digital polymerase chain reaction (dPCR) assay, the device comprising:

• an inlet opening;
• an outlet opening;
• a flow channel connecting said inlet opening and said outlet opening and defining a flow direction from the inlet opening through the flow channel to the outlet opening, wherein the flow channel comprises a first flow channel surface and a second flow channel surface opposite to the first flow channel surface;
• and
• a honeycomb structured array of hexagonal shaped wells provided in said first flow channel surface for fluidic communication with the inlet opening and the outlet opening, wherein each of the hexagonal shaped wells comprises a vertex oriented in the flow direction facing towards the side of the inlet opening, further wherein the hexagonal shape of each well comprises a hexagon elongated in the flow direction such that a length of the well is elongated relative to the width of the well;
• wherein the first flow channel surface provides a first hydrophilicity and at least a part of the second flow channel surface provides a second hydrophilicity that is less than said first hydrophilicity.