Methods for Localization of Cancerous Tissue Using Fluorescent Molecular Imaging Agent for Diagnosis or Treatment

A method comprising:

• navigating an instrument, via a minimally invasive route, into a human patient to whom a molecular imaging agent has been intravenously administered, to position the instrument in an area of a tissue abnormality;
• and
• visualizing, via the instrument, tissue in the area under near-infrared (NIR) light, wherein the molecular imaging agent, as administered, causes abnormal tissue in the area to fluoresce under the NIR light and enable the fluorescing abnormal tissue to be localized within the area,
• wherein the molecular imaging agent comprises VGT-309 or a pharmaceutically acceptable salt thereof, and
• wherein the molecular imaging agent is administered to the patient at a dose from 0.01 mg/kg to 0.7 mg/kg between 2 hours and 4 days prior to the navigating.