Biosynthesis of cannabinoids and cannabinoid precursors
AssigneeGinkgo Bioworks, Inc.
InventorBrian Carvalho
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Abstract
Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.
Claims118
A method for producing a cannabinoid compound, comprising contacting olivetol and geranyl pyrophosphate with a prenyltransferase (PT), wherein the PT comprises an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO:
- 34.
A method for producing a cannabinoid compound, comprising culturing a host cell in the presence of olivetol, wherein the host cell comprises a heterologous polynucleotide encoding a prenyltransferase (PT), wherein the PT comprises an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO:
- 34.
A method for producing a cannabinoid compound, comprising contacting cannabigerol (CBG) with a terminal synthase (TS), wherein the TS comprises an amino acid sequence that is at least 90% identical to the sequence of any one of SEQ ID NOs:
- 27, 38, 44, and 50.
A method for producing a cannabinoid compound, comprising culturing a host cell in the presence of cannabigerol (CBG), wherein the host cell comprises a heterologous polynucleotide encoding a TS, wherein the TS comprises an amino acid sequence that is at least 90% identical to the sequence of any one of SEQ ID NOs:
- 27, 38, 44, and 50.
A composition comprising olivetol and a heterologous polynucleotide encoding a prenyltransferase (PT), wherein the PT comprises an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO:
- 34, and wherein the PT is capable of utilizing olivetol as a substrate for producing cannabigerol (CBG).
A host cell that comprises olivetol and a heterologous polynucleotide encoding a prenyltransferase (PT), wherein the PT comprises an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO:
- 34, and wherein the host cell is capable of producing cannabigerol (CBG).
A composition comprising cannabigerol (CBG) and a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS is a fungal TS, and wherein TS is capable of producing cannabichromene (CBC).
A composition comprising cannabigerol (CBG) and a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises an amino acid sequence that is at least 90% identical to the sequence of any one of SEQ ID NOs:
- 27, 38, 44, and 50, wherein the TS is capable of utilizing CBG as a substrate to produce a cannabinoid compound.
A bioreactor for producing a cannabinoid compound, wherein the bioreactor contains olivetol and a prenyltransferase (PT) comprising an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO:
- 34.
A bioreactor for producing a cannabinoid compound, wherein the bioreactor contains CBG and a terminal synthase (TS) comprising a sequence that is at least 90% identical to the sequence of any one of SEQ ID NOs:
- 27, 38, 44, and 50.
A bioreactor for producing a cannabinoid compound, wherein the bioreactor contains:
- (i) a prenyltransferase (PT) comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 34;
- and
- (ii) a terminal synthase (TS) comprising a sequence that is at least 90% identical to the sequence of any one of SEQ ID NOs:
- 27, 38, 44, and 50.
Dependencies are inferred from the claim text.