US 2024/0384285 A1Published application

Transgenic plants producing high levels of apocarotenoids compounds and uses thereof

AssigneesUniversidad De Castilla La Mancha, Consejo Superior de Investigaciones Cientificas, Universitat Politècnica de València

InventorsMaría Lourdes GÓMEZ GÓMEZ, Oussama AHRAZEM EL KADIRI, Antonio GRANELL RICHART, Ángela RUBIO MORAGA, Javier ARGANDOÑA PICAZO, Silvia PRESA, Jose Luis RAMBLA

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Abstract

Methods and materials for recombinantly producing apocarotenoids from transgenic plants, and more particularly, tomato and potato plants, expressing heterologous genes from Crocus sativus, the saffron plant, under the control of specific promoters. Moreover, the genetic constructs including the heterologous genes are also related, and the use thereof for the expression in plants and to a method for obtaining transgenic plants which express the genetic construct mentioned above, and which have a high concentration of apocarotenoids products, in particular crocins and picrocrocins. Furthermore, the the food products obtained from such transgenic plants are also related, nutraceutical and pharmaceutical compositions including thereof and the use of such compositions as medicine, preferably for the treatment of neurodegenerative diseases such as Alzheimer's disease.

Claims15

Dependent claims

A DNA construct comprising nucleotide sequences encoding for a combination of three proteins for apocarotenoids compounds biosynthesis, preferably crocins, crocetins, picrocrocin, HTTC and safranal, wherein the proteins are selected from UGT2, UGT709G1 and CCD2L, or a functionally equivalent sequence thereof, and wherein these proteins belong to genus Crocus,

wherein the amino acid sequences for UGT2, UGT709G1 and CCD2L are selected from an amino acid sequence at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the sequences selected from the list consisting of:
SEQ ID NO:
4, SEQ ID NO:
6 and SEQ ID NO:
2 respectively,
wherein each nucleotide sequences being operably linked to a promoter selected from a fruit specific promoter, tuber specific promoter and/or a constitutive promoter, and
wherein the nucleotide sequence encoding for the CCD2L is operably linked to a fruit specific promoter or a tuber specific promoter.

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