Bioluminescence-triggered photocatalytic activation

A method of proximity-dependent activation of a molecule within a cell, comprising contacting a cell with a luminophore under conditions in which the luminophore enters the cell, wherein the cell comprises:

• (a) a fusion of a bioluminescent protein and a capture protein, wherein the bioluminescent protein catalyzes emission of a first wavelength of light from the luminophore upon interaction therewith;
• (b) a conjugate of (A) a capture ligand and (B) a photocatalyst or photosensitizer, wherein the capture protein forms a covalent bond with the capture ligand upon interaction therewith, and wherein the photocatalyst or photosensitizer is activated by exposure to light of the first wavelength;
• and
• (c) an activatable molecule, wherein the activatable molecule is converted into an activated molecule when in proximity to the activated photocatalyst or photosensitizer.

A method of proximity-dependent activation of an activatable molecule within a cell, comprising:

• (a) expressing a fusion of a bioluminescent protein and a capture protein within the cell;
• (b) contacting the cell with a luminophore, under conditions in which the luminophore enters the cell, wherein the bioluminescent protein catalyzes emission of a first wavelength of light from the luminophore upon interaction therewith;
• (c) contacting the cell with a conjugate of (i) a capture ligand and (ii) a photocatalyst or photosensitizer, under conditions in which the conjugate enters the cell, wherein the capture protein forms a covalent bond with the capture ligand upon interaction therewith, and wherein the photocatalyst or photosensitizer is activated by exposure to light of the first wavelength;
• and
• (d) contacting the cell with an activatable molecule, wherein the activatable molecule is converted into an activated molecule when in proximity to the activated photocatalyst or photosensitizer.

A method of proximity-dependent activation of a photocatalyst or photosensitizer within a cell, comprising:

• (a) expressing a fusion of a bioluminescent protein and a capture protein within the cell;
• (b) contacting the cell with a luminophore, under conditions in which the luminophore enters the cell, wherein the bioluminescent protein catalyzes emission of a first wavelength of light from the luminophore upon interaction therewith;
• and
• (c) contacting the cell with a conjugate of (i) a capture ligand and (ii) a photocatalyst or photosensitizer, under conditions in which the conjugate enters the cell, wherein the capture protein forms a covalent bond with the capture ligand upon interaction therewith, and wherein the photocatalyst or photosensitizer is activated by exposure to light of the first wavelength.