Nanopore sensor devices
AssigneeIllumina, Inc.
InventorsBoyan Boyanov, Jeffrey G. Mandell, Seth M. McDonald
Life of this patent
Estimating the term…
Abstract
An example of a nanopore sensor device includes one or more cis wells; a cis electrode; a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/solid-state membrane having a nanopore; a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells; a first concentration of an electrolyte within the one or more cis wells; and a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration.
Claims21
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/polymer/solid-state membrane having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- a first concentration of an electrolyte within the one or more cis wells;
- and
- a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration.
A nanopore sensor kit, comprising:
- a nanopore sensor device, including:
- one or more cis wells including a fluid inlet;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/polymer/solid-state membrane having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- and
- a first concentration of an electrolyte within the one or more cis wells and the plurality of trans well;
- and
- a second concentration of the electrolyte to be introduced into the one or more cis wells through the fluid inlet such that the one or more cis wells contain the second concentration of the electrolyte and the plurality of trans wells contain the first concentration of the electrolyte at an initial cycle of the nanopore sensor device, wherein the second concentration is higher than the first concentration.
A method of detecting an ionic current to analyze a biological compound, comprising:
- providing a nanopore within a membrane separating a cis well and a trans well, the nanopore having a plurality of positively charged residues on an inner surface;
- providing an electrolyte within the cis well and the trans well;
- and
- applying an electric current between a cis cathode at least partially exposed to the cis well and a trans anode at least partially exposed to the trans well to generate an ionic current through the nanopore,
- wherein the plurality of positively charged residues of the nanopore inhibits translocation of cations from the trans well to the cis well during application of the electric current.
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/polymer/solid-state membrane having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- an electrolyte solution including:
- a redox-inactive buffer that includes a redox inactive species having a diameter greater than a diameter of a constriction zone of the nanopore;
- and
- a redox couple.
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/polymer/solid-state membrane having a nanopores with a plurality of positively charged residues on an inner surface of the nanopore;
- and
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells.
Dependencies are inferred from the claim text.