Non-canonical lipoproteins with programmable assembly and architecture
AssigneeSyracuse University
InventorDavoud Mozhdehi
Life of this patent
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Abstract
Artificial lipoproteins bearing non-canonical post-translational modifications that are synthesized by leveraging substrate promiscuity of an acyltransferase. The non-canonical functionality of these lipoprotein results in a distinctive hysteretic assembly that is absent from the canonical lipoproteins and is used to prepare hybrid multiblock materials with precise and programmable patterns of amphiphilicity due to the unique assembly and function of the non-canonical post-translational modifications.
Claims18
A non-naturally occurring lipoprotein, comprising an intrinsically disordered peptide polymer fused to a non-natural fatty acid having the formula R-COOH, where R contains a linear chain of no more than 15 atoms.
A method of synthesizing a non-naturally occurring lipoprotein, comprising the steps of:
- modifying a host organism to express N-myristoyl transferase;
- coexpressing N-myristoyl transferase and an intrinsically disordered peptide polymer fused to a peptide substrate of the lipid modification enzyme from the host organism;
- allowing post-translational modification by the host organism in the presence of an expression media containing a non-natural fatty acid;
- and
- isolating the non-naturally occurring lipoprotein resulting from coexpressing N-myristoyl transferase and the intrinsically disordered peptide polymer fused to the peptide substrate in the presence of the non-natural fatty acid.
Dependencies are inferred from the claim text.