Methods and systems for detecting peptide aggregates
AssigneeRensselaer Polytechnic Institute
InventorPeter M. Tessier
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Abstract
Provided herein are conformation-specific antibodies which bind to protein aggregates, such as amyloid fibrils, including aggregates of glucagon and liraglutide. Also provided are methods of using the antibodies to detect protein aggregates in samples, and methods for identifying aggregate-binding antibodies.
Claims20
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A method for identifying one or more antibodies which bind preferentially to a protein in aggregate form compared with a monomeric form, the method comprising:
- (a) performing one or more negative screens of an antibody library against a monomeric form of the protein,
- (b) performing one or more positive screens of the antibody library against aggregate forms of the protein, and
- (c) repeating steps (a) and (b), whereby one or more antibodies are identified that bind to the protein in aggregate form with high affinity and bind to monomers of the same protein with low affinity.
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Dependencies are inferred from the claim text.