Methods for making flow cells

A method, comprising:

• introducing a functional material into a flow cell including two patterned substrates, each of the patterned substrates including a resin layer having depressions separated by interstitial regions, wherein:
• the resin layers include an ultraviolet (UV) light blocking additive;
• the depressions of each of the two patterned substrates overlie a first portion of the resin layer having a first thickness and the interstitial regions of each of the two patterned substrates overlie a second portion of the resin layer having a second thickness that is greater than the first thickness;
• and
• the functional material is susceptible to interaction with the resin layer of each of the two patterned substrates when exposed to UV light;
• and
• simultaneously directing, through the resin layer of each of the two patterned substrates, a predetermined UV light dosage, whereby some of the functional material within the depressions of a first of the two patterned substrates is exposed to the predetermined UV light dosage and attaches to the resin layer within the depressions of the first of the two patterned substrates, some other of the functional material within the depressions of a second of the two patterned substrates is exposed to the predetermined UV light dosage and attaches to the resin layer within the depressions of the second of the two patterned substrates, and the functional material in contact with the interstitial regions of each of the two patterned substrates is blocked from being exposed to the predetermined UV light dosage by the second resin portion.

A method, comprising:

• depositing a first functional material over a resin layer including depressions separated by interstitial regions, wherein:
• the resin layer includes an ultraviolet (UV) light blocking additive;
• the depressions overlie a first portion of the resin layer having a first thickness and the interstitial regions overlie a second portion of the resin layer having a second thickness that is greater than the first thickness;
• and
• the first functional material is susceptible to interaction with the resin layer when exposed to UV light;
• directing, through the resin layer, a first predetermined UV light dosage, whereby the first functional material within the depressions is exposed to the first predetermined UV light dosage and attaches to the resin layer within the depressions, and the first functional material overlying the interstitial regions is blocked from being exposed to the first predetermined UV light dosage by the second resin portion and remains unattached to the resin layer at the interstitial regions;
• removing the first functional material from the interstitial regions;
• depositing a second material over the interstitial regions and over the first functional material in the depressions, wherein the second material is susceptible to interaction with the resin layer when exposed to UV light and is not susceptible to interaction with the first functional material when exposed to UV light;
• and
• directing a second predetermined UV light dosage directly at the second material, whereby the second material is exposed to the second predetermined UV light and attaches to the resin layer at the interstitial regions and remains unattached to the first functional material.