Method and system for spectral imaging
AssigneePentaomix Ltd.
InventorBoaz BRILL
Life of this patent
Estimating the term…
Abstract
A method of imaging a sample comprises serially illuminating the sample by a plurality of light beams, each having a different central wavelength. The method also comprises: serially acquiring from the sample image data by an imager, wherein the image data represent optical signals received from the sample responsively to the plurality of light beams. The method also comprises shifting a field-of-view of the sample relative to the imager, repeating the serial illumination and the image data acquisitions for the shifted field-of-view, and generating a spectral image of the sample using image data acquired by the imager at a plurality of field-of-views for each of the plurality of light beams.
Claims33
A method of imaging a sample, comprising:
- serially illuminating the sample by a plurality of light beams, each having a different central wavelength;
- by an imager, serially acquiring from the sample image data representing optical signals received from the sample responsively to said plurality of light beams;
- shifting a field-of-view of the sample relative to said imager and repeating said serial illumination and said image data acquisitions for said shifted field-of-view;
- and
- generating a spectral image of the sample using image data acquired by said imager at a plurality of field-of-views for each of said plurality of light beams.
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A system for imaging a sample, comprising:
- an illumination system configured for serially illuminating the sample by a plurality of light beams, each having a different central wavelength;
- an imager, configured for acquiring image data from the sample, said image data representing optical signals received from the sample responsively to said plurality of light beams;
- a stage configured for shifting a field-of-view of the sample relative to said imager;
- a controller, configured to control said stage to shift said field-of-view in steps, and to control said illumination system and said imager such said illumination system serially illuminates the sample by said light beams and said imager serially acquires said image data;
- and
- an image processor configured to generate a spectral image of the sample using image data acquired by said imager at a plurality of field-of-views for each of said plurality of light beams.
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A Sagnac interferometer, comprising:
- two attached prisms forming an asymmetric monolithic structure having an entry facet at one prism and an exit facet at another prism;
- and
- a beam splitter, engaging a portion of an attachment area between said prisms and being configured for splitting an optical signal entering through said entry facet into two secondary optical signals exiting through said exit facet;
- wherein a size of said beam splitter is selected to ensure that optical paths of said secondary optical signals impinge on said attachment area both at locations engaged by said beam splitter and at locations not engaged by said beam splitter.
Dependencies are inferred from the claim text.