Cell-free bioproduction of b-cryptoxanthin and zeaxanthin
AssigneeDebut Biotechnology, Inc.
InventorsDREW WAGNER, Nicholas Brideau, Joshua Britton
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Abstract
A cell-free system is provided to produce β-cryptoxanthin and zeaxanthin from β-carotene. β-carotene is added to a reaction mixture comprising a β-carotene hydroxylase enzyme (CrtZ). The reaction mixture may be aqueous and may comprise a co-solvent, e.g., an organic co-solvent, such as THF.
Claims30
A method for cell-free production of hydroxylated carotenoid, wherein the method comprises transformation of a substrate through an enzyme to hydroxylated carotenoid.
A composition for cell-free production of hydroxylated carotenoid by enzymatic transformation of a substrate to a hydroxylated carotenoid, wherein the composition comprises a substrate and an enzyme.
Dependencies are inferred from the claim text.