Microbial cell factories producing vitamin b compounds

The host cell of claims 24 to 41, wherein the one or more pathway elements are encoding by one or more genes selected from the group of:

• a) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 2 or genomic DNA thereof encoding the BioC comprised in SEQ ID NO:
• 1;
• b) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 4 or genomic DNA thereof encoding the BioH comprised in SEQ ID NO:
• 3;
• c) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 6 or genomic DNA thereof encoding the BioF comprised in SEQ ID NO:
• 5;
• d) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 8 or genomic DNA thereof encoding the BioA comprised in SEQ ID NO:
• 7;
• e) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 10 or genomic DNA thereof encoding the Biok comprised in SEQ ID NO:
• 9;
• f) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 12 or genomic DNA thereof encoding the BioD comprised in SEQ ID NO:
• 11;
• g) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 14 or genomic DNA thereof encoding the Biol comprised in SEQ ID NO:
• 13;
• h) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 16 or genomic DNA thereof encoding the BioW comprised in SEQ ID NO:
• 15;
• i) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 18, 20, 22, 24, 26, 28, 30, 32, 34, or 36 or genomic DNA thereof encoding the IscR comprised in SEQ ID NO:
• 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35 respectively;
• and
• j) a gene which is at least 50%, such as at least 60%, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% identical to the gene comprised in SEQ ID NO:
• 38 or genomic DNA thereof encoding the BioB comprised in SEQ ID NO:
• 37.

A mutated a polypeptide selected from the group consisting of a polypeptide which is at least 90% identical to:

• a) the mutant CRP having a sequence comprised in SEQ ID NO:
• 39 or 97 and further comprising one or more mutations in positions corresponding to T12, D138, T146, F69, R82, V139, G57, K58, E59, M60, 161, L62, S63, G72, E73, L74, R83, S84, T128, S129, A136, F137, Q171, E172, 1173, G174, Q175, 1176, V177, G178, C179, S180, R181, E182, T183, V184, G185, and/or R186 of SEQ ID NO:
• 39 OR 97;
• b) the mutant CRR having a sequence comprised in SEQ ID NO:
• 41 or 99 and further comprising one or more mutations in positions corresponding to H76 and/or H91 of SEQ ID NO:
• 41 OR 99;
• and/or
• c) the mutant CyaA having a sequence comprised in SEQ ID NO:
• 43 or 101 and further comprising one or more mutations in positions corresponding to G60, K59, L63, T65, R188, G195, K196, R192, S103, S113, D114, D116, W118, E185, T189, K260, K264, K332, W200, and/or D300 of SEQ ID NO:
• 43 OR 101.