Methods to produce therapeutic formulations comprising hydroxybutirate and hydroxyvalerate, therapeutic formulations and uses thereof

A process for producing optically active (R)-3-hydroxybutyrate and (R)-3-hydroxyvalerate mixtures from biomass containing poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHB-co-HV), comprising:

• a) adjusting the 3-hydroxyvalerate content in the PHB-co-HV polymer between 0 and 40%, by controlled addition of sugars and one or more precursor compounds in a fermentation phase of the PHB-co-HV;
• b) extracting PHB-co-HV from biomass by the addition of one or more proteolytic enzymes;
• c) adding an alcohol and an acidifying agent to the extracted PHB-co-HV to form a suspension;
• d) heating the suspension to a temperature of 70 to 150° C. to form a solution;
• e) neutralizing the formed solution with an alkalizing agent in a reaction;
• f) distilling the mixture of (R)-3-hydroxybutyrate/(R)-3-hydroxyvalerate esters under vacuum to remove excess alcohol and form a distilled mixture;
• g) adding an alkalizing agent to the distilled mixture;
• and
• h) removing volatile compounds by vacuum distillation, and collecting the final mixture (R)-3-hydroxybutyrate and (R)-3-hydroxyvalerate.