Systems, compositions, and methods for biocatalyzed production of a grease thickener
AssigneeExxonMobil Technology and Engineering Company
InventorsXiaozhou ZHANG, Jay PATEL
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Abstract
Systems, methods, and compositions for biocatalyzed conversion of oleic acid to non-racemic (R)-10-hydroxystearic acid ((R)-10-HSA) are described herein. Some systems and methods may include mixing oleic acid, a buffer, and a surfactant to produce an emulsion. The emulsion may be mixed with recombinant enzyme produced by a transformed microorganism. The recombinant enzyme may biocatalytically convert oleic acid to (R)-10-HSA, which may be extracted from the emulsion via an organic solvent.
Claims22
A biocatalyzed method for the conversion of oleic acid to non-racemic (R)-10-hydroxystearic acid (10-HSA) comprising:
- mixing oleic acid, a buffer, and a surfactant to produce an emulsion;
- mixing the emulsion with an enzyme having at least 85% homology with any of SEQ ID. No. 19-35 to produce a catalyzed emulsion;
- extracting non-racemic (R)-10-HSA from the catalyzed emulsion using an organic solvent;
- and
- at least partially isolating at least a portion of the non-racemic (R)-10-HSA from the organic solvent.
A bacterial cell including at least one recombinant plasmid including a region having at least 85% homology with any of SEQ IDs. No. 1-17.
A composition comprising a recombinant nucleic acid having at least 85% homology with any of SEQ ID. No. 1-17.
A composition comprising recombinant protein having at least 85% homology with any of SEQ ID. No. 19-35.
Dependencies are inferred from the claim text.