Genetically modified yeast and fermentation processes for the production of lactate
AssigneeCargill, Incorporated
InventorsKeith Michael BRADY, Hans H. LIAO
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Abstract
Disclosed herein are genetically engineered yeast cells capable of producing lactate from sucrose. The genetically engineered yeast cells comprise a polynucleotide encoding an exogenous lactate dehydrogenase enzyme: a polynucleotide encoding an exogenous invertase enzyme: a deletion or disruption of a native pyruvate decarboxylase (PDC) gene: and a genetic modification resulting in overexpression of a native hexokinase gene.
Claims33
A genetically engineered yeast cell capable of producing lactate from sucrose, the engineered yeast cell comprising
- a polynucleotide encoding an exogenous lactate dehydrogenase enzyme;
- a polynucleotide encoding an exogenous invertase enzyme comprising a sequence at least 80% identical to at least one of SEQ ID NOs:
- 12, 33, 34, and 35;
- a deletion or disruption of a native pyruvate decarboxylase (PDC) gene;
- and
- a genetic modification resulting in overexpression of a native hexokinase gene.
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A method for producing lactate from sucrose, the method comprising:
- contacting a substrate comprising sucrose with an engineered yeast cell comprising
- a polynucleotide encoding an exogenous lactate dehydrogenase enzyme;
- a polynucleotide encoding an exogenous invertase enzyme;
- a deletion or disruption of a native pyruvate decarboxylase (PDC) gene;
- and
- a genetic modification resulting in overexpression of a native hexokinase gene,
- wherein fermentation of the substrate by the engineered yeast produces lactate.
32-37. (canceled)
A genetically engineered Issatchenkia orientalis cell capable of producing lactate from sucrose, the engineered yeast cell comprising
- a polynucleotide encoding an exogenous lactate dehydrogenase enzyme;
- a polynucleotide encoding an exogenous invertase enzyme;
- a deletion or disruption of a native pyruvate decarboxylase (PDC) gene;
- and
- a genetic modification resulting in overexpression of a native hexokinase gene, wherein the engineered I. orientalis cell is capable of producing lactate at a titer of at least 30.
Dependencies are inferred from the claim text.