Preparatory process
AssigneeTelix Pharmaceuticals (Innovations) Pty Ltd.
InventorsOliver William STERRITT, Michael Paul WHEATCROFT
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Abstract
The disclosure provides a process of preparing an iron complexed conjugate between a desferrioxamine chelating ligand and a protein, the process being undertaken at a substantially neutral pH. A process of exposing an iron complexed conjugate to a free chelating ligand selected from desferrioxamine, a desferrioxamine analogue, and HBED, at a substantially neutral pH, in order to remove chelated iron from the iron complexed conjugate is also disclosed.
Claims23
A process for preparing a conjugate of a desferrioxamine chelating ligand linked with a protein, the process comprising exposing an iron complexed conjugate comprising the desferrioxamine chelating ligand complexed to iron linked with the protein, to a free chelating ligand selected from desferrioxamine, a desferrioxamine analogue and HBED, or a combination thereof, at substantially neutral pH to thereby remove iron from the desferrioxamine chelating ligand of the iron chelated conjugate and provide the conjugate of the desferrioxamine chelating ligand linked with the protein that is substantially free of chelated iron.
A process of preparing a conjugate of a desferrioxamine chelating ligand linked with a protein, the process comprising combining at substantially neutral pH:
- a first composition comprising an iron chelated desferrioxamine chelating ligand and a polar organic solvent and
- a second composition comprising a protein,
- to thereby form an iron complexed conjugate of the desferrioxamine chelating ligand complexed to iron linked with the protein.
A process of preparing a conjugate of a desferrioxamine chelating ligand linked with a protein, the process comprising:
- conjugating an iron chelated desferrioxamine chelating ligand with a protein to provide an iron complexed conjugate of the desferrioxamine chelating ligand complexed to iron linked with the protein;
- and
- exposing the iron complexed conjugate comprising the desferrioxamine chelating ligand complexed to iron linked with the protein, to a free chelating ligand selected from desferrioxamine, a desferrioxamine analogue and HBED, or a combination thereof, at substantially neutral pH to thereby remove iron from the desferrioxamine chelating ligand of the iron chelated conjugate and provide the conjugate of the desferrioxamine chelating ligand linked with the protein that is substantially free of chelated iron.
Dependencies are inferred from the claim text.