Configurable platform

A system for fluorescence imaging of an object, comprising:

• an image sensor assembly comprising at least one image sensor;
• and
• an optical assembly configured to transmit light emitted from the object to the image sensor assembly, the optical assembly comprising at least one notch filter configured to block light in a plurality of fluorescence excitation wavebands while transmitting fluorescence light that is emitted from the object,
• wherein the optical assembly is configured to project the emitted fluorescence light as one or more fluorescence images onto the at least one image sensor of the image sensor assembly.

A method for fluorescence imaging, comprising:

• generating, by an illuminator of an imaging system, light in a first excitation waveband to cause a first fluorescence light emission from a first target;
• blocking, by at least one notch filter of the imaging system, the light in the first excitation waveband while transmitting the first fluorescence light emission to at least one image sensor of the imaging system;
• generating, by the illuminator, light in a second excitation waveband to cause a second fluorescence light emission from a second target;
• and
• blocking, by the at least one notch filter of the imaging system, the light in the second excitation waveband while transmitting the second fluorescence light emission to the at least one image sensor of the imaging system.