Systems and methods for analysis of peptide photodissociation for singlemolecule protein sequencing
AssigneeBrown University
InventorsDerek M. Stein, Jacob Sage Vietorisz, Nicholas Drachman
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Abstract
The present disclosure generally relates to systems and methods for analysis of peptide photodissociation for single-molecule protein sequencing. In one aspect, systems and methods are directed to allowing one to fragment a single protein molecule in aqueous solution so that its composition and sequence of amino acids can be measured by mass spectrometry. This may be useful for single-molecule protein sequencing technology.
Claims32
1-25. (canceled)
A method comprising:
- arranging a protein into a substantially linear configuration in a nanotip;
- fragmenting the protein into amino acids by applying laser light to the protein;
- emitting the amino acids from the nanotip;
- and
- detecting the amino acids emitted from the nanotip.
27-30. (canceled)
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46-61. (canceled)
A mass spectrometer, comprising:
- an ion source comprising a capillary;
- a light source directed towards the ion source, wherein the light source is able to produce light at a wavelength of greater than or equal to 150 nm and less than or equal to 213 nm;
- a magnetic mass filter downstream of the ion source;
- an array of detectors downstream of the magnetic mass filter.
67-73. (canceled)
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79-83. (canceled)
A method of sequencing a protein, comprising:
- passing a fluid comprising a protein into a capillary defining an opening;
- applying light at a wavelength of greater than or equal to 150 nm and less than or equal to 213 nm to the protein proximate the opening to produce fragments;
- passing the fragments directly into an environment having a pressure of no more than 100 mPa;
- passing the fragments through a magnetic mass filter;
- directing the fragments to an array of detectors;
- and
- determining a sequence of the protein by determining the fragments with the array of detectors.
85-87. (canceled)
Dependencies are inferred from the claim text.