Blood cell analyzer, method for indicating intection status and use of infection marker parameter

A method of using an infection marker parameter in evaluating an infection status of a subject, wherein the infection marker parameter is obtained by:

• calculating at least one first leukocyte parameter of at least one first target particle population obtained by flow cytometry detection of a first test sample containing a part of a blood sample to be tested from the subject, a first hemolytic agent, and a first staining agent for leukocyte classification;
• by flow cytometry detection of a second test sample containing another part of the blood sample to be tested, a second hemolytic agent, and a second staining agent for identifying nucleated red blood cells, wherein at least one of the first leukocyte parameter and the second leukocyte parameter comprises a cell characteristic parameter;
• and
• calculating the infection marker parameter based on the at least one first leukocyte parameter and the at least one second leukocyte parameter.

A blood cell analyzer, comprising:

• a sample aspiration device configured to aspirate a blood sample of a subject to be tested;
• a sample preparation device configured to prepare a first test sample containing a first part of the blood sample to be tested, a first hemolytic agent, and a first staining agent for leukocyte classification, and to prepare a second test sample containing a second part of the blood sample to be tested, a second hemolytic agent and a second staining agent for identifying nucleated red blood cells;
• an optical detection device comprising a flow cell, a light source and an optical detector, wherein the flow cell is configured to allow the first test sample and the second test sample to pass therethrough respectively, the light source is configured to respectively irradiate with light the first test sample and the second test sample passing through the flow cell, and the optical detector is configured to detect first optical information and second optical information generated by the first test sample and second test sample under irradiation when passing through the flow cell respectively;
• and
• a processor configured to:
• calculate at least one first leukocyte parameter of at least one first target particle population in the first test sample from the first optical information,
• calculate at least one second leukocyte parameter of at least one second target particle population in the second test sample from the second optical information, wherein at least one of the first leukocyte parameter and the second leukocyte parameter comprises a cell characteristic parameter,
• calculate an infection marker parameter for evaluating an infection status of the subject based on the at least one first leukocyte parameter and the at least one second leukocyte parameter, and
• output the infection marker parameter.