Transgene expression of ligninolytic enzymes in fungi
AssigneeBattelle Memorial Institute
InventorsZiyu Dai, Ana L. Robles, Joonhoon Kim, Scott E. Baker, Jon K. Magnuson, Beth A. Hofstad, John M. Gladden
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Abstract
Fungus with incorporated of heterologous ligninolytic enzyme to enhance effective conversion of lignocellulosic feedstocks to various chemical productions.
Claims26
A modified filamentous fungus transformed with at least two polynucleotides coding for manganese peroxidase (mnP), versatile peroxidase, laccase or lignin peroxidase (lip).
A method to increase expression of ligninolytic enzyme from a filamentous fungus comprising transforming said fungus with at least two polynucleotides encoding manganese peroxidase (mnP), versatile peroxidase, laccase or lignin peroxidase (lip) and culturing said transformed fungus so as to express at least two of manganese peroxidase (mnP), versatile peroxidase, laccase or lignin peroxidase (lip).
The method of 13 comprising, further culturing said transformed fungus in culture medium comprising a protein selected from hemoglobin, soy protein, skim milk protein, serum albumin or combination thereof.
Dependencies are inferred from the claim text.