Method for simultaneous and efficient detection of residues of 36 antidepressants in sludges

A method for simultaneous and efficient detection of residues of 36 antidepressants in sludge, comprising the following steps:

• step 1, extraction of target drugs:
• weighing a sludge sample, and then performing solvent extraction to obtain an extraction solution containing the antidepressants;
• step 2, purification of the extraction solution:
• purifying the extraction solution;
• step 3, detection of the purified extraction solution by a technique of ultra-performance liquid chromatography-tandem mass spectrometry;
• and
• step 4:
• qualitative and quantitative analysis of the antidepressants;
• wherein the 36 antidepressants comprise agomelatine, alprazolam, amitriptyline, bupropion, buspirone, carbamazepine, chlordiazepoxide, clobazam, clonazepam, clozapine, desvenlafaxine, diazepam, doxepin, duloxetine, escitalopram, estazolam, flunitrazepam, fluoxetine, flurazepam, imipramine, lamotrigine, mianserin, midazolam, mirtazapine, moclobemide, nitrazepam, nordazepam, nortriptyline, prazepam, quetiapine, reboxetine, ropivacaine, sertraline, tandospirone, trazodone, and venlafaxine;
• the step of performing solvent extraction to obtain the extraction solution containing the antidepressants comprises the following steps:
• freeze-drying the sludge sample, grinding and sieving the freeze-dried sludge, weighing the sludge sample, successively adding an inorganic extractant and an organic extractant to the sludge sample, mixing a resultant evenly, and then performing ultrasonic and centrifugal extraction to collect a supernatant, wherein the inorganic extractant is an aqueous ammonia solution, and the organic extractant is one or more of methanol, ethyl acetate and n-hexane;
• and
• the step of purifying the extraction solution comprises:
• passing the extraction solution collected in step 1 through pass-through EMR-LPD cartridges containing Carbon S, collecting a purified effluent, drying the purified effluent with nitrogen at room temperature, redissolving the dried effluent in methanol, mixing a resultant evenly, and filtering a resulting mixture to collect a filtrate, wherein the filtrate is preserved in the dark for later on-instrument detection.