Biomarkers to detect risk of sinusoidal obstruction syndrome
AssigneeMUSC Foundation for Research Development
InventorSOPHIE PACZESNY
Life of this patent
Estimating the term…
Abstract
The present disclosure provides biomarker panels for evaluating subjects at risk of sinusoidal obstruction syndrome (SOS) early after hematopoietic stem cell transplantation (HSCT). In particular, the present disclosure relates to the use of ST2. L-Ficolin, and HA for prognosing, diagnosing, and/or treating SOS.
Claims27
A method of treating and/or preventing sinusoidal obstructive syndrome (SOS) in a subject receiving hematopoietic stem cell transplantation (HCT), said the method comprising:
- (a) obtaining a biological sample from a subject receiving HCT;
- (b) measuring in said biological sample from the subject the expression of L-Ficolin, hyaluronic acid (HA), and IL-1RL1 (ST2) by contacting the biological sample obtained from the subject with specific binding agents that specifically bind to each of the respective the biomarkers, wherein each specific binding agent forms a complex with the respective biomarker;
- (c) detecting the agent-biomarker complexes, thereby determining the biomarker expression level, wherein decreased expression level of L-ficolin, elevated expression level of HA, and/or elevated expression level of ST2 as compared to a control identifies a subject at risk of SOS;
- and
- (d) treating said patient at risk of SOS with a therapeutic agent.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
24-27. (canceled)
An in vitro method for measuring the expression of the antigens L-ficolin, HA, and ST2 comprising:
- (a) obtaining a sample from a subject having received a hematopoietic stem cell transplantation (HCT);
- (b) contacting said sample with an anti-L-ficolin antibody, an anti-HA antibody, and an anti-ST2 antibody to form antigen-antibody complexes;
- and
- (c) detecting the antigen-antibody complexes using detectable moieties that distinctly bind each of the antibodies, thereby measuring the expression of the antigens L-ficolin, HA, and ST2 in said sample.
30-49. (canceled)
Dependencies are inferred from the claim text.