Electrophoretic Bar Code Assay Devices and Methods for Making and Using the Same
AssigneeThe Regents of the University of California
InventorsM. Kursad Araz, Akwasi A. Apori, Amy E. Herr
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Abstract
A microfluidic device for determining whether an analyte is present in a sample is provided. The microfluidic device includes an elongated flow path having a polymeric medium, where the polymeric medium includes a first analyte detection domain having a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain having a second immobilized capture member that specifically binds to a second analyte. Also provided are methods, systems and kits in which the subject microfluidic devices find use, as well as methods of producing the same.
Claims18
A microfluidic device comprising:
- an elongated flow path comprising a polymeric medium, wherein the polymeric medium comprises a first analyte detection domain comprising a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain comprising a second immobilized capture member that specifically binds to a second analyte.
A method of determining whether an analyte is present in a sample, the method comprising:
- introducing a sample into an elongated flow path comprising a polymeric medium, wherein the polymeric medium comprises a first analyte detection domain comprising a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain comprising a second immobilized capture member that specifically binds to a second analyte;
- applying a directional electric field to the elongated flow path in a manner sufficient to move components through the polymeric medium;
- and
- obtaining a signal from the first analyte detection domain and the second analyte detection domain to determine whether the first analyte and the second analyte are present in the sample.
-21. (canceled)
Dependencies are inferred from the claim text.