Conductive polymer with covalent capture ability and methods of using same
AssigneeDuke University
InventorsMichael Tadross, Isaac Weaver
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Abstract
Disclosed herein are electrically conductive polymer compositions comprising a polymer and a dispersant, and methods of synthesizing the polymers therein. Also disclosed herein are microelectrode arrays comprising the electrically conductive material and a conductive polymer composition attached to a surface of the electrically conductive material. Further disclosed herein are methods of detecting electrical activity in a cell comprising contacting the microelectrode array with a cell and detecting electrical activity in the cell by covalently attaching the polymer of the conductive polymer composition to a protein on the surface of the cell.
Claims31
A compound of formula (I)
- or a salt thereof, wherein:
- L is alkylene, heteroalkylene, heteroalkylene-C(O)NRa-heteroalkylene, heteroalkylene-C(O)O-heteroalkylene, heteroalkylene-OC(O)NRa-heteroalkylene, alkylene-C(O)—, heteroalkylene-C(O)—, heteroalkylene-O(O)NRa, heteroalkylene-OC(O)NRa, heteroalkylene-C(O)O-heteroalkylene-C(O)—, heteroalkylene-C(O)NR3-heteroalkylene-C(O)—, or heteroalkylene-OC(O)NR1-heteroalkylene-C(O)—;
- Z is a binding ligand selected from the group consisting of HaloTag, SNAP-tag, TMP-tag, βLac-tag, and CLIP-tag;
- and
- Ra is hydrogen or alkyl.
A conductive polymer composition comprising a polymer and a dispersant, the polymer including recurring units of formula (II)
- wherein:
- R1 is hydrogen or
- L is alkylene, heteroalkylene, heteroalkylene-C(O)NRa-heteroalkylene, heteroalkylene-C(O)O-heteroalkylene, heteroalkylene-OC(O)NRa-heteroalkylene, alkylene-C(O)—, heteroalkylene-C(O)—, heteroalkylene-C(O)NRa, heteroalkylene-OC(O)NRa, heteroalkylene-C(O)O-heteroalkylene-C(O)—, heteroalkylene-C(O)NRa-heteroalkylene-C(O)—, or heteroalkylene-OC(O)NRa-heteroalkylene-C(O)—;
- Ra is hydrogen or alkyl;
- Z is a binding ligand selected from the group consisting of HaloTag, SNAP-tag, TMP-tag, βLac-tag, and CLIP-tag;
- and
- q is 1 to 10,000,000.
A method of electrochemically synthesizing a conductive polymer, the method comprising
- adding a first mixture to an electrically conductive material, the first mixture comprising a solvent, a polystyrene sulfonate (PSS), and a 3,4-ethylenedioxythiophene (EDOT);
- applying an electrical current to the electrically conductive material to provide a poly(3,4-ethylenedioxythiophene) (PEDOT) on a surface of the electrically conductive material;
- adding a second mixture to the electrically conductive material, the second mixture comprising a solvent, a dispersant having a molecular weight of less than 1 kDa, and a compound of formula (I)
- or a salt thereof, wherein:
- L is alkylene, heteroalkylene, heteroalkylene-C(O)NRa-heteroalkylene, heteroalkylene-C(O)O-heteroalkylene, heteroalkylene-OC(O)NRa-heteroalkylene, alkylene-C(O)—, heteroalkylene-C(O)—, heteroalkylene-C(O)NRa, heteroalkylene-OC(O)NRa, heteroalkylene-C(O)O-heteroalkylene-C(O)—, heteroalkylene-C(O)NRa-heteroalkylene-C(O)—, or heteroalkylene-OC(O)NRa-heteroalkylene-C(O)—;
- Z is a binding ligand selected from the group consisting of HaloTag, SNAP-tag, TMP-tag, βLac-tag, and CLIP-tag;
- and
- Ra is hydrogen or alkyl;
- and
- applying an electrical current to the electrically conductive material to provide a second polymer attached to the PEDOT, the second polymer including recurring units of formula (II-b)
- wherein:
- L is alkylene, heteroalkylene, heteroalkylene-C(O)NRa-heteroalkylene, heteroalkylene-C(O)O-heteroalkylene, heteroalkylene-OC(O)NRa-heteroalkylene, alkylene-C(O)—, heteroalkylene-C(O)—, heteroalkylene-C(O)NRa, heteroalkylene-OC(O)NRa, heteroalkylene-C(O)O-heteroalkylene-C(O)—, heteroalkylene-C(O)NRa-heteroalkylene-C(O)—, or heteroalkylene-OC(O)NRa-heteroalkylene-C(O)—;
- Z is a binding ligand selected from the group consisting of HaloTag, SNAP-tag, TMP-tag, βLac-tag, and CLIP-tag;
- Ra is hydrogen or alkyl;
- and
- z is 1 to 10,000,000.
Dependencies are inferred from the claim text.