METHODS AND SYSTEMS FOR ISOLATING VERY SMALL EMBRYONIC STEM CELLS (VSELs) FROM BLOOD

A method for isolation very small embryonic stem cells from peripheral blood of a small animal, the method comprising:

• obtain a blood sample from the small animal;
• position the blood sample in a first centrifuge tube and a second centrifuge tube;
• insert a first centrifuge tube and a second centrifuge tube in a centrifuge, and spin the centrifuge tube at 250 relative centrifugal force at a temperature above freezing and below ten degrees temperature;
• remove plasma from the first centrifuge tube and second centrifuge tube, and position the removed plasma in a third centrifuge tube;
• run plasma within the third centrifuge tube through a filter to remove white blood cells, larger stem cells, and residual red blood cells from the plasma;
• run the remaining plasma in a third centrifuge tube at 1300 relative centrifugal force to isolate stem cell pellets at the bottom of the third centrifuge tube;
• and
• position plasma without the stem cell pellets in a fourth centrifuge tube, and put the fourth centrifuge tube ultra-sonification bath to lysate platelets.