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AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsEun Jig Lee, Daham Kim
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The present invention relates to an autoimmune disease animal model and a method for preparing the same. The animal model of the present invention efficiently generates an autoantibody that causes autoimmune disease, by introducing an autoantigen-encoding gene through the Cre-LoxP system, which enables stable and continuous expression of the autoantigen at a desired time by treatment of Cre recombinase. Contrary to the conventional method that fails to effectively produce autoantibody due to leaky expression and immune tolerance, or requires repeated administration over several weeks, the present invention achieves sustainable expression of an effective amount of autoantigen by only one TAT-Cre recombinase treatment, thus may be utilized as an outstanding animal model reproducing various symptoms and molecular mechanisms of autoimmune diseases.
A gene delivery system for preparing an animal in which an autoimmune disease is induced, comprising in a 5′ to 3′ direction, an expression regulatory sequence, an expression blocking nucleotide with loxp (locus of X-over P1) nucleotide sequence flanking on both sides, and a nucleotide encoding an autoantigen protein.
An animal in which an autoimmune disease is induced, comprising an expression blocking nucleotide with loxp (locus of X-over P1) nucleotide sequence flanking on both sides, and a nucleotide encoding an autoantigen protein.
Dependencies are inferred from the claim text.