Wheat with reduced susceptibility to late-maturity alpha-amylase
AssigneesCommonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation
InventorsJean-Philippe RAL, Crispin HOWITT, Jos MEOG, Michelle COLGRAVE, James BROADBENT, Uptal BOSE, Steve JOBLING
Life of this patent
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Abstract
The present disclosure relates generally to wheat plants comprising a genetic modification leading to reduced expression of at least one Amy 1 gene on chromosome 6B. The present disclosure further relates to grain from such plants and to products derived from the grain.
Claims57
A wheat plant comprising a genetic modification leading to reduced expression of at least one Amy1 gene on chromosome 6B relative to a wild-type wheat plant, wherein grain from the plant is characterized by a reduced level or activity, or both, of alpha-amylase 1 (AMY1) polypeptides relative to a wild-type wheat grain.
Wheat grain comprising a genetic modification leading to reduced expression of at least one Amy1 gene on chromosome 6B relative to a wild-type wheat grain, wherein the grain is characterized by a reduced level or activity, or both, of AMY1 polypeptides relative to a wild-type wheat grain.
A nucleic acid molecule which encodes an AMY1 polypeptide, wherein the nucleic acid molecule differs from the nucleic acid of SEQ ID NO:
- 13, 17, 21, 25 and/or 29, or a nucleic acid sequence which is at least about 91% identical to the nucleic acid sequence of SEQ ID NO:
- 13, 17, 21, 25 and/or 29, by a mutation selected from a deletion mutation, an insertion mutation, a splice-site mutation, a premature translation termination mutation, a nonsense mutation and a frameshift mutation.
An AMY1 polypeptide, wherein the polypeptide differs from the amino acid sequence of SEQ ID NO:
- 16, 20, 24, 28 and/or 32, or an amino acid sequence which is at least 99% identical to the amino acid sequence of SEQ ID NO:
- 16, 20, 24, 28 and/or 32, by at least one amino acid substitution, insertion or deletion in the active site, catalytic site, calcium binding site and/or carbohydrate binding domain, wherein the AMY1 polypeptide has an impaired structure and/or reduced activity relative to an AMY1 polypeptide having the amino acid sequence of SEQ ID NO:
- 16, 20, 24, 28 and/or 32.
A method of producing a plant with reduced susceptibility to LMA, the method comprising:
- a. introducing a genetic modification to at least one Amy1 gene on chromosome 6B to a plant cell such that the cell has reduced level or activity, or both, of AMY1 polypeptides relative to unmodified cells;
- b. regenerating a plant with the genetic modification from the cell of step (a), wherein the plant has reduced susceptibility to LMA relative to wild-type plants lacking the genetic modification.
A method of determining susceptibility to LMA in a wheat plant, the method comprising:
- a. obtaining a sample of grain from the wheat plant;
- b. analyzing the sample for the presence of at least one AMY1 peptide selected from the amino acid sequences of SEQ ID NOs:
- 59-93, or an amino acid sequence which comprises not more than three modifications to an amino acid sequence of SEQ ID NOs:
- 59-93;
- and/or at least one AMY2 peptide selected from the amino acid sequences of SEQ ID NOs:
- 94-103, or an amino acid sequence which comprises not more than three modifications to an amino acid sequence of SEQ ID NOs:
- 94-103;
- c. comparing the peptides analyzed in step (b) to those present in control wheat grain, and based on this comparison, determining if the wheat plant is susceptible to LMA.
The method of any one of claims 47 to 53, wherein the AMY1 peptides comprising the amino acid sequences of SEQ ID NOs:
- 59-93 and the AMY2 peptides comprising the amino acid sequences of SEQ ID NOs:
- 94-103 are analyzed in step (b).
Dependencies are inferred from the claim text.