Biosensors for the detection of antigens using antibody fragments
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsAlexander Star, Wenting Shao
Life of this patent
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Abstract
A sensor device for detecting an antigen in a fluid sample includes a sensor including a substrate and a sensor medium on the substrate. The sensor medium includes a plurality of single-walled carbon nanotubes having an enriched semiconducting content and one or more antibody fragments immobilized on the plurality of single-walled carbon nanotubes. Each of the one or more antibody fragments includes an active binding site for the antigen. The sensor device further includes electronic circuitry including at least one measurement system in operative connection with the sensor to measure a variable providing a measure of change in at least one property of the sensor medium which is dependent upon the presence of the antigen.
Claims21
A sensor device for detecting an antigen in a fluid sample, comprising:
- a sensor comprising a substrate and a sensor medium on the substrate, the sensor medium including a plurality of single-walled carbon nanotubes having an enriched semiconducting content and one or more antibody fragments immobilized on the plurality of single-walled carbon nanotubes, each of the one or more antibody fragments comprising an active binding site for the antigen, and electronic circuitry including at least one measurement system in operative connection with the sensor to measure a variable providing a measure of change in at least one property of the sensor medium which is dependent upon the presence of the antigen.
A sensor, comprising:
- a substrate and a sensor medium on the substrate, the sensor medium comprising a plurality of single-walled carbon nanotubes having an enriched semiconducting content and one or more antibody fragments immobilized on the plurality of single-walled carbon nanotubes, each of the one or more antibody fragments comprising an active binding site for an antigen to be detected, wherein at least one property of the sensor medium is dependent upon the presence of the antigen.
A method of detecting an antigen in a fluid sample, comprising:
- providing a sensor device including a sensor including a substrate and a sensor medium on the substrate, the sensor medium including a plurality of single-walled carbon nanotubes having an enriched semiconducting content and one or more antibody fragments, each of the one or more antibody fragments comprising an active binding site for the antigen, and electronic circuitry including at least one measurement system in operative connection with the sensor to measure a variable providing a measure of change in at least one property of the sensor medium which is dependent upon the presence of the antigen;
- exposing the sensor to the fluid sample for a period of time;
- and
- measuring an output of the sensor with a liquid of known ionic strength over the sensor medium.
Dependencies are inferred from the claim text.