Back to results
US 2024/0344984 A1Published application
Multi-dimensional widefield infrared-encoding spontaneous emission (“md-wise”) microscopy
AssigneeThe Regents of the University of California
InventorsChang YAN, Wei XIONG
Life of this patent
Estimating the term…
Abstract
A multiplexed widefield imaging method employs spatially focusing femtosecond infrared (IR) pulses and visible pulses delayed by a controlled temporal delay onto a sample stained with one or more chromophore to excite spontaneous emitted photoluminescence (PL) signals from the chromophores. The resulting PL signals are detected to generate PL images in which intensities of the PL signals are a function of optical frequencies of the IR and visible pulses and the temporal delay.
Claims22
A multiplexed widefield imaging method comprising:
- spatially focusing femtosecond infrared (IR) pulses and visible pulses delayed by a controlled temporal delay onto a sample comprising at least one chromophore to excite spontaneous emitted photoluminescence (PL) signals from the at least one chromophore;
- and
- detecting at a detector the PL signals to generate PL images, wherein intensities of the PL signals within the PL images are a function of optical frequencies of the IR and visible pulses and the temporal delay.
A method for distinguishing chromophores in a sample comprising:
- spatially focusing femtosecond IR and visible pulses delayed by a controlled temporal delay onto the sample stained with chromophores to excite spontaneous emitted PL signals;
- detecting at a detector the PL signals to generate a PL image, wherein intensities within the PL image are a function of optical frequencies of the IR and visible pulses and the temporal delay.
Dependencies are inferred from the claim text.