Self-replicating dna expression system and immunogen
OwnerEdward Peter Rybicki, Guy Louis Regnard +1inventors
InventorsEdward Peter Rybicki, Guy Louis Regnard, Warren Ralph Josephus De Moor
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Abstract
The present invention relates to self-replicating DNA expression vectors comprising elements from Beak and Feather Disease Virus (BFDV), including a BFDV LIR element comprising an origin of replication (Ori), genes encoding a BFDV Rep protein and a heterologous polypeptide of interest under the control of a mammalian promoter, wherein the Rep protein is capable of binding to the Ori to initiate replication of the expression vector. Pharmaceutical compositions and cells comprising the self-replicating vectors are also provided. The self-replicating vectors may be used in methods of inducing an immune response or expressing a polypeptide in a subject, or as an expression system.
Claims22
A self-replicating DNA expression vector comprising:
- a) at least one BFDV LIR element comprising an origin of replication (Ori);
- b) a gene encoding a BFDV replication-associated protein (Rep);
- c) a gene encoding a heterologous polypeptide of interest;
- and
- d) a mammalian promoter operably linked to (b) and (c),
- wherein the Rep protein is capable of binding to the Ori to initiate replication of the expression vector.
-18. (canceled)
Dependencies are inferred from the claim text.