Prokaryotic and eukaryotic cells with biosynthesized sulfotyrosine for genetic incorporation
AssigneeWilliam Marsh Rice University
InventorsHan XIAO, Yuda CHEN
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Abstract
The present disclosure provides an engineered cell comprising a sulfotransferase. In some embodiments, the sulfotransferase is NnSULT1C1 sulfotransferase. The present disclosure also provides methods for the biosynthesis of a peptide containing at least one sulfotyrosine residue, as well as compositions comprising a peptide containing at least one sulfotyrosine residue. In some embodiments, the compositions and methods of the present disclosure provide therapeutic peptides for use in treating and/or preventing a disease or disorder, such as an HIV-1 infection.
Claims29
An engineered cell comprising a sulfotransferase having a sequence that is at least about 95% identical to the amino acid sequence of SEQ ID NO:
- 1.
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A method of expressing a recombinant peptide comprising at least one sulfotyrosine residue at a selected position not found in a wild-type version of the peptide, the method comprising:
- a. obtaining an engineered cell that comprises 3′-phosphoadenosine-5′-phosphosulfate and a sulfotransferase that is at least about 95% identical to SEQ ID NO:
- 1;
- b. expressing a nucleic acid encoding the recombinant peptide in the cell;
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- c. purifying the recombinant peptide from the cell.
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Dependencies are inferred from the claim text.