Anti-csf1r car expressing lymphocytes for targeted tumor therapy

A CAR comprising an extracellular domain that specifically binds CSF1R, a transmembrane domain, and an intracellular T cell activating domain, said extracellular domain comprising

• (i) an antigen binding region that is a human or humanized scFv antigen binding region specific for CSF1R;
• and
• (ii) a spacer comprising a human hinge region and a detectable tag allowing the detection and/or purification of said CAR or a cell expressing said CAR which spacer does not comprise an antibody Fc region or portion thereof and/or does not have binding activity for one or more Fc receptors;
• wherein said intracellular T cell activating domain comprises the signaling domain of the CD3ζ chain and/or at least one costimulatory domain that is an intracellular domain of an endogenous T cell receptor.

A method for producing a lymphocyte recombinantly expressing a chimeric antigen T cell receptor (CAR), the method comprising

• (a) introducing into the lymphocyte a polynucleotide encoding the CAR, or an expression vector comprising said polynucleotide;
• (b) culturing the lymphocyte recombinantly engineered according to (a) under conditions allowing the expression of the CAR;
• and
• (c) recovering the engineered lymphocyte;
• wherein the CAR comprises an extracellular domain that specifically binds CSF1R, a transmembrane domain, and an intracellular T cell activating domain, said extracellular domain comprising
• (i) an antigen binding region that is a human or humanized scFv antigen binding region specific for CSF1R;
• and
• (ii) a spacer comprising a human hinge region and a detectable tag allowing the detection and/or purification of said CAR or a cell expressing said CAR which spacer does not comprise an antibody Fc region or portion thereof and/or does not have binding activity for one or more Fc receptors:
• wherein said intracellular T cell activating domain comprises the signaling domain of the CD3ζ chain and/or at least one costimulatory domain that is an intracellular domain of an endogenous T cell receptor.