Methods and kits for detecting cannabinoids
AssigneesRicovr Healthcare Inc., Indian Institute of Technology Madras (Iit Madras)
InventorsVenkata Raghavendra Sai Vemulakonda, Divagar Murugan, Himanshu Bhatia, Joseph Walter Seimetz, Elana Michelle Edery
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Abstract
The disclosure provides methods of detecting a cannabinoid or metabolite thereof in a sample and test kits for the same. For example, the cannabinoid such as tetrahydrocannabinol (THC) bound to bovine serum albumin (BSA) may induce changes in optical properties of an optical biosensor employed for the said detection wherein the optical biosensor is immobilized with a suitable bioreceptor or through interactions with a bioreceptor-nanoparticle conjugate.
Claims15
A method of detecting presence or absence of a cannabinoid or metabolite thereof in a biological sample, comprising:
- contacting the biological sample with nanoparticle conjugated bioreceptor(s) capable of binding the cannabinoid or metabolite thereof, wherein
- the nanoparticle conjugated bioreceptor(s) are either immobilized on a fiber optic sensor, or
- the nanoparticle conjugated bioreceptor(s) are in solution phase;
- and
- detecting a change in optical property of the fiber optic sensor in response to
- direct binding of the cannabinoid or metabolite thereof to the nanoparticle conjugated bioreceptor(s) immobilized on the fiber optic sensor surface, or
- binding of free nanoparticle conjugated bioreceptor(s) in solution phase to cannabinoid or metabolite thereof immobilized on a fiber optic sensor surface.
Dependencies are inferred from the claim text.