Cancer risk assessment
AssigneeMicromass UK Limited
InventorsJohannes Vissers, Billy J. Molloy, Dominic Foley
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to methods of quantifying a target protein e.g., a tumor biomarker, in a sample by using a mass spectroscopy coupled with a chromatography method. The methods presented herein include incubating the sample with a composition comprising an amidase and a protease for a predetermined amount of time. Incubating the sample with a composition comprising an amidase and a protease in a single step improves the digestion efficiency of the target protein, leading to enhanced detectability e.g., signal to noise ratio, in mass spectroscopy.
Claims13
A method of quantifying a target protein in a sample, the method comprising the steps of:
- a. incubating the sample with a composition comprising an amidase and a protease for a predetermined amount of time;
- b. subjecting the sample to a chromatography method;
- and
- c. quantifying the target protein using a mass spectroscopy method.
Dependencies are inferred from the claim text.