Chimeric pseudotyped recombinant rabies virus
AssigneeBeam Therapeutics Inc.
InventorsChieYu Lin, Fei Ran, Apurva Govande
Life of this patent
Estimating the term…
Abstract
Provided herein are chimeric pseudotyped recombinant RABV particles, wherein the chimeric envelope protein comprises, from N-terminus to C-terminus, a non-RABV envelope protein ectodomain, a non-RABV envelope protein transmembrane domain, and a RABV C-terminal domain. Also provided are methods for their use in delivering a transgene into a target cell.
Claims33
A pseudotyped recombinant rabies virus (RABV) particle, comprising a chimeric envelope protein and a recombinant RABV genome, wherein:
- the chimeric envelope protein comprises, from N-terminus to C-terminus, a non-RABV envelope protein ectodomain, a non-RABV envelope protein transmembrane domain, and a RABV C-terminal domain;
- and
- the recombinant RABV genome does not encode an endogenous envelope protein or fragment thereof and comprises a nucleic acid encoding a therapeutic transgene.
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The pseudotyped particle of claim 14, wherein the Rhabdoviridae family virus comprises an ephemerovirus, a tibrovirus, an almendravirus, a novirhabdovirus, a tupavirus, a nonvirhabdovirus, or a moussa virus (MOUV), optionally wherein the ephemerovirus is bovine ephemeral fever virus (BEFV);
- the tibrovirus is an ekpoma virus 1 (EKV1) or an ekpoma virus 2 (EKV2);
- the tibrovirus is a Bas-Congo tibrovirus (BASV);
- the tibrovirus is a tibrogargan virus (TIBV);
- the almendravirus is arboretum virus (ABTV) or balsa virus (BALV);
- the nonvirhabdovirus is viral hemorrhagic septicemia virus (VSHV);
- or
- the tupavirus is tupaia Virus (TUPV).
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Dependencies are inferred from the claim text.