Culture medium, coating matrix and method for expanding midbrain dopaminergic progenitor cells
AssigneeNuwacell Biotechnologies Co., Ltd.
InventorsJunying YU, Luyan JIAO, Yongqiang ZHAO, Ying ZHANG
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Abstract
The present disclosure described herein provides, inter alia, an expansion method for expanding mDAPs and a culture medium and a coating matrix combination used in the expansion method, as well as a maturation method for maturing mDAPs and a culture medium used in the maturation method. The present disclosure also provides a substantially homogeneous population of mDAPs and a substantially homogeneous population of mDANs.
Claims16
1-30. (canceled)
A culture medium that promotes the expansion of midbrain Dopaminergic Progenitor cells (mDAPs), said culture medium comprising:
- (a) a basal medium;
- (b) a neural growth supplement;
- (c) a WNT signaling pathway activator;
- (d) a Rho Kinase (ROCK) inhibitor;
- and
- (e) a Transforming Growth Factor β (TGF-β) inhibitor.
Dependencies are inferred from the claim text.