Cell culture membrane and method for producing biological tissue

A method for producing a biological tissue including a plurality of cell layers, the method comprising:

• supplying cells and a culture solution to two culture spaces of a biological tissue forming device including a cell culture membrane and the two culture spaces, the cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane being disposed between a plurality of cell layers after the cells are cultured, and the cell culture membrane further containing methyl cellulose having a weight average molecular weight of from 20000 to 190000 as a main component, and the two culture spaces being divided by the cell culture membrane;
• culturing the cells in the two culture spaces to form cell layers on both surfaces of the cell culture membrane;
• and
• dissolving methyl cellulose in the cell culture membrane at 15° C. or lower.

A method for producing a biological tissue including a plurality of cell layers, the method comprising:

• supplying cells and a culture solution to two culture spaces of a biological tissue forming device including a cell culture membrane and the two culture spaces, the cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane being disposed between a plurality of cell layers after the cells are cultured, the cell culture membrane containing methyl cellulose having a weight average molecular weight of from 190000 to 400000 as a main component, and the cell culture membrane further containing a dissolution temperature regulator, and the two culture spaces being divided by the cell culture membrane;
• culturing the cells in the two culture spaces to form cell layers on both surfaces of the cell culture membrane;
• and
• dissolving methyl cellulose in the cell culture membrane at 20° C. or lower.