Methods and compositions for treating disorders associated with bile acids
AssigneeNovome Biotechnologies, Inc.
InventorsPeter Spanogiannopoulos, Weston Robert Whitaker, Kathy Lam, Jessica Tan, Zachary Nicholas Russ, William Cain DeLoache, Lauren Popov, Lane Weaver
Life of this patent
Estimating the term…
Abstract
The disclosure relates generally to bacteria that have been modified to metabolize a bile acid, pharmaceutical compositions including the bacteria, and methods of using the bacteria and pharmaceutical compositions to treat disorders associated with an elevated amount of bile acid, e.g., bile acid diarrhea.
Claims24
(canceled)
A bacterium comprising one or more transgenes that increase or enable the bacterium's ability to metabolize one or more bile acids or bile salts, wherein the one or more transgenes comprise:
- (i) a transgene encoding a 3α-hydroxysteroid dehydrogenase (3α-HSDH), or a functional fragment or variant thereof, having at least 80% identity to an amino sequence encoded by SEQ ID NO:
- 18;
- and/or
- (ii) a transgene encoding a 3β-hydroxysteroid dehydrogenase (3β-HSDH), or a functional fragment or variant thereof, having at least 80% identity to an amino sequence encoded by any one of SEQ ID NOs:
- 28, 30, 32, or 36.
-137. (canceled)
A method of reducing a level of a bile acid in a subject, the method comprising administering to the subject a bacterium comprising one or more transgenes that increase the bacterium's ability to metabolize the bile acid, wherein the one or more transgenes comprise:
- (i) a transgene encoding a 3α-hydroxysteroid dehydrogenase (3α-HSDH), or a functional fragment or variant thereof, having at least 80% identity to an amino sequence encoded by SEQ ID NO:
- 18;
- and/or
- (ii) a transgene encoding a 3β-hydroxysteroid dehydrogenase (3β-HSDH), or a functional fragment or variant thereof, having at least 80% identity to an amino sequence encoded by any one of SEQ ID NOs:
- 28, 30, 32, or 36.
139-143. (canceled)
A method of treating a bile acid disorder in a subject in need thereof, the method comprising administering to the subject a bacterium comprising one or more transgenes that increase the bacterium's ability to metabolize one or more bile acids, wherein the one or more transgenes comprise:
- (i) a transgene encoding a 3α-hydroxysteroid dehydrogenase (3α-HSDH), or a functional fragment or variant thereof, having at least 80% identity to an amino sequence encoded by SEQ ID NO:
- 18;
- and/or
- (ii) a transgene encoding a 3β-hydroxysteroid dehydrogenase (3β-HSDH), or a functional fragment or variant thereof, having at least 80% identity to an amino sequence encoded by any one of SEQ ID NOs:
- 28, 30, 32, or 36.
145-158. (canceled)
Dependencies are inferred from the claim text.