Recombinant production of cannabinoids

A recombinant organism configured to produce a cannabinoid, comprising:

• a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM);
• wherein:
• the eukaryotic organism includes Pichia pastoris;
• the geranyl diphosphate synthase (GPPS2) is derived from Abies grandis, the isopentenyl diphosphate isomerase (IDI) is derived from E. coli, the isopentenyl phosphate kinase (IPK) is derived from Methanothermobacter, and the 5-(hydroxyethyl)-methyl thiazole kinase (ThiM) is derived from E. coli;
• the recombinant construct further includes prenyltransferase (NphB) derived from Streptomyces;
• the recombinant construct is incorporated into the genomic DNA of the recombinant organism;
• and
• the recombinant organism includes a Ku70 gene knockout.