Novel method for producing poly-4-hydroxybutyrate and 1,4-butanediol
AssigneeCj Cheiljedang Corporation
InventorsSeo Hyoung KIM, Kyung-Chang LEE, Jae Hyung LIM, Hyo-Seok HA, Dong-Eun CHANG
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Abstract
The present disclosure relates to a novel method of producing poly-4-hydroxybutyrate and/or 1,4-butanediol, and a microorganism using a poly-4-hydroxybutyrate production pathway.
Claims38
A method of producing 1,4-butanediol, the method comprising:
- (1) converting succinyl-CoA (SuCoA) to succinate semialdehyde (SSA);
- (2) converting succinate semialdehyde (SSA) to 4-hydroxybutyrate (4HB);
- (3) converting 4-hydroxybutyrate (4HB) to 4-hydroxybutyryl CoA (4HBCoA);
- (4) producing poly-4-hydroxybutyrate (P4HB) by polymerizing two or more of 4-hydroxybutyryl CoA (4HBCoA);
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- (5) degrading poly-4-hydroxybutyrate to 1,4-butanediol.
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A microorganism comprising succinate semialdehyde dehydrogenase, 4-hydroxybutyric acid dehydrogenase, 4-hydroxybutyryl-CoA transferase, and poly(3-hydroxyalkanoate) polymerase polypeptide, a polynucleotide encoding the polypeptide, or a combination thereof.
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Dependencies are inferred from the claim text.