Method for producing plasmid dna using escherichia coli
AssigneesKaneka Corporation, Kaneka Eurogentec S.A., Université Catholique De Louvain
InventorsTomohisa Tokunaga, Tozo Nishiyama, Kazunobu Minakuchi, Seung Hyun Cho, Jean-Francois Collet, Michael Deghelt
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Abstract
Plasmid DNA can be efficiently recovered from bacterial cells when producing plasmid DNA using E. coli. Provided is a method for producing plasmid DNA including the following steps (a) to (c): (a) a step of preparing E. coli, which has a mutation in a gene region associated with maintaining outer membrane properties and has a desired plasmid; (b) a step of culturing E. coli of (a); and (c) a step of recovering a desired plasmid from bacterial cells after culture.
Claims20
A method for producing plasmid DNA, comprising the following steps (a) to (c):
- (a) a step of preparing E. coli, which has a mutation in a gene region associated with maintaining outer membrane properties and has a desired plasmid;
- (b) a step of culturing E. coli of (a);
- and
- (c) a step of recovering a desired plasmid from bacterial cells after culture.
A method for preparing E. coli for plasmid DNA production, comprising the steps (i) and (ii):
- (i) a step of causing a mutation in a gene region associated with maintaining outer membrane properties of E. coli;
- and
- (ii) a step of preparing E. coli, which has the mutation obtained in (i) and has a desired plasmid.
E. coli for producing plasmid DNA, which has a mutation in a gene region associated with maintaining outer membrane properties and has a desired plasmid.
Dependencies are inferred from the claim text.