Methods for oxyfunctionalization of various substrates using bacterial enzymes

Abstract

The invention relates to the field of protein engineering and biocatalysis, in particular to methods for oxygenation of aliphatic alkenes and terpenes using bacterial enzymes. Provided is a method for oxyfunctionalization of a substrate of interest, comprising contacting an aliphatic alkene or a terpene substrate with a source of hydrogen peroxide and a polypeptide having calcosin-like peroxy genase activity (EC 1.11.2.1), wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 50% pairwise sequence identity when aligned to at least 150 consecutive amino acid residues of Seq. No. 2 shown in Table 1, and comprising at least the following heme-coordinating motifs: i) HXXFFD: ii) H(X)XD, wherein X is any amino acid; and (b) a fragment of the polypeptide of (a) that has calcosin-like peroxygenase activity.

Dependencies are inferred from the claim text.