Structurally complete organoids
AssigneeChildren's Hospital Medical Center
InventorsJames Macormack Wells, Alexandra Kay Eicher
Life of this patent
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Abstract
Disclosed herein are compositions of gastrointestinal organoids comprising cells originating from all three primary germ layers and methods of making and use thereof. These gastrointestinal organoids exhibit complex cellular organization and functions resembling naturally occurring organ tissue, and serve as excellent three dimensional models for studying gastrointestinal physiology.
Claims56
A method for preparing a gastrointestinal organoid from the three primary germ layers, comprising:
- contacting gut endoderm spheroids with splanchnic mesoderm cells (SM) and enteric neural crest cells (ENCCs) to form a cell mixture;
- and
- culturing the cell mixture under conditions sufficient to differentiate the cell mixture into a gastrointestinal organoid comprising epithelium, mesenchyme, and a functional enteric nervous system (ENS).
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Dependencies are inferred from the claim text.